系別：化工系
課名：生物技術 課號：524 U0860 學分數：3
授課教師：黃世佑、謝學真

一、課程內容：
1. Introduction
- Recent advances in biotechnology
- Applications in healthcare, agriculture, food and chemical
industries
- Stem cell and gene therapy
2. Molecular and Cellular Biology
- Molecules of life
- Cell structure and functions
- Signal transduction
- Cell cycle and its control
3. Gene Manipulation
- Transcription and translation
- Control of gene expression
- Recombinant DNA technology
- DNA cloning
- Gene expression systems
- Applications
4. Enzyme Technology, Enzyme Immobilization
- Enzyme properties, enzymatic processes
- Immobilized-enzyme technology
- Some commercial enzyme applications
5. Biosensors and Biochips
- Basic elements of biosensors
- Some typical biosensors
- Some examples and principle of biochips
6. Microbial Processes
- Classification of microbes, microbial world
- Some traditional and modern fermentation processes
- Metabolic control in the fermentation processes
7. Animal Cell Culture Engineering
- Culture environment and cell growth
- Scale-up of animal cell culture
- Animal cell products
- Tissue engineering
8. Immunotechnology
- Immune system and antibodies
- Hybridoma and monoclonal antibodies
- Applications
9. Plant Biotechnology: Tissue and Cell Cultures
- Principle and technology in plant tissue/cell cultures
- Production of valuable secondary metabolites
- Transgenesis of plant cells and its applications
- Somatic embryogenesis of plant cells

二、參考書籍：
1. Smith, J. E.: Biotechnology, 3rd ed., Cambridge University
Press, Cambridge, 1996.
2. Primrose, S. B: Molecular Biotechnology, 2nd ed., Blackwell
Scientific Publications, Oxford, 1991.
3. Alberts, B. et al.: Molecular Biology of the Cell, 4th ed.,
Garland Science, New York, 2002.
4. Eggins, B. R.: Biosensors: An Introduction, John Wiley, New
York, 1996.
5. Old, R. W. and Primrose, S. B.: Principles of Gene Manipulation,
5th ed., Blackwell Scientific, Oxford, 1994.
6. Smith, C. A. and Wood, E. J.: Molecular Biology and
Biotechnology, 1st ed., Chapman & Hall, London, 1991.
7. Freshney, R. I.: Culture of Animal Cells, 4th ed., Wiley-Liss,
New York, 2000.

三、成績評量方式：期中考、期末考、期末報告等

四、預修課程:生物學、微生物學、生物化學等相關之課程（含通識）